Ducati's Casey Stoner finished yesterday's practice session on top by over a second over Fiat Yamaha's Valentino Rossi who came in second at the Brno circuit.

Bridgestone riders Toni Elias, Shinya Nakano, and Chris Vermeulen finished the day's running third, fourth, and fifth fastest respectively for Ducati, Honda, and Suzuki, ahead of Colin Edwards, who was first of the Michelin runners on his Tech 3 Yamaha in sixth.

Pos Rider Bike Time
1. Casey Stoner Ducati 1:56.261
2. Valentino Rossi Yamaha 1:57.332
3. Toni Elias Ducati 1:57.457
4. Shinya Nakano Honda 1:57.652
5. Chris Vermeulen Suzuki 1:57.742
6. Colin Edwards Yamaha 1:57.756
7. Alex de Angelis Honda 1:57.826
8. Sylvain Guintoli Ducati 1:57.859
9. Jorge Lorenzo Yamaha 1:57.979
10. Loris Capirossi Suzuki 1:58.177
11. Niccolo Canepa Ducati 1:58.204
12. Marco Melandri Ducati 1:58.325
13. Randy De Puniet Honda 1:58.568
14. Dani Pedrosa Honda 1:59.067
15. Olivier Jacque Kawasaki 1:59.130
16. Andrea Dovizioso Honda 1:59.130
17. James Toseland Yamaha 1:59.228
18. John Hopkins Kawasaki 2:00.414
19. Tadyuki Okada Honda 2:00.735